Mouse T Cell Enrichment Kit

Unwanted cells are targeted for removal with tetrameric antibody complexes recognizing non t cells and dextran coated magnetic particles.

Mouse t cell enrichment kit. This kit uses the new fast mouse protocol and replaces the cd4 t cell isolation kit ii 130 095 248 allowing isolation of cd4 t cells in just 18 minutes. If cells are more dilute than 10 x 10 6 cells ml then spin down the cells and resuspend them in tissue culture medium or 1x bd imag buffer at a concentration of 20 x 10 6 cells ml. Cells were stained with cd8 clone 53 6 7 pe and cd3ε clone 145 2c11 apc. This product replaces the easysep mouse cd4 t cell enrichment kit catalog 19752 for even faster cell isolations.

This product replaces the easysep mouse t cell enrichment kit catalog 19751 for even faster cell isolations. Add the bioinylated mouse cd8 t lymphocyte enrichment cocktail at 5 µl per 1 x 10 6 cells and incubate on ice for 15 minutes 5. The magnisort mouse t cell enrichment kit is designed for the magnetic separation of mouse t cells by negative selection. The cd4 t cell isolation kit mouse was developed for the untouched isolation of cd4 t cells from single cell suspensions of mouse spleen and or lymph nodes.

It has been optimized for the isolation of mouse t cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin coated magnetic beads. T cell enrichment column kits are also available to isolate specific t cell subpopulations of interest. Available for human mouse and rat cells purifies cd3 t cells to 81 88 based upon gravitational flow simple procedure. The easysep human t cell enrichment kit is designed to isolate t cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection.

Easysep mouse cd4 t cell isolation kit catalog 19852. The easysep human cd8 t cell enrichment kit is designed to isolate cd8 t cells from fresh or previously frozen peripheral blood mononuclear cells or ammonium chloride lysed leukapheresis sample by negative selection. Fast and easy to use up to 99 purity no columns required untouched viable cells. Before loading onto t cell subset columns leukocyte suspensions are incubated with a mixture of monoclonal antibodies.

A single cell suspension from pooled c57bl 6 mouse peripheral lymphoid tissues was prepared to isolate cd8 t cells using the mojosort mouse cd8 t cell isolation kit. Fast and easy to use up to 96 purity no columns required untouched viable cells. Designed to prepare purified murine t cell populations via high affinity negative selection. The resulting column eluate is a highly enriched t cell population with minimal depletion of t cell constituents.